Riccia fluitans: From Tissue Culture to Farm
Paper ID : 1020-ICIAQUA
Authors
Afzan Muntaziana Mohd Pazai *, Sharifah Nurul Nabila Syed Mohamad, Norhanizan Sahidin
Department of Fisheries Malaysia
Abstract
Purpose: The application of tissue culture in aquatic plants presents promising avenues for sustainable production. The objective of this paper is to investigate whether Riccia fluitans can be directly cultivated from tissue culture stock to the greenhouse without a period of acclimatization in a lab setting, aiming for mass propagation for commercial purposes
Method: Riccia fluitans were sub-cultured in MS food medium for 2 months. The plants were carefully removed from the media and affixed onto stainless steel mesh panels measuring 8cm x 8cm. 5 treatments were conducted: (T1) Control treatment cultivated the specimen in a laboratory, utilizing a closed container (T2) Cultivated in the greenhouse, utilizing an open container, (T3) Cultivated in the greenhouse, utilizing a closed container, (T4) Specimen involved pre-acclimatizing period for a month in a laboratory setting before cultivated them in greenhouse, utilizing an open container and (T5) Specimen involved pre-acclimatizing period for a month in a laboratory setting before cultivated them in greenhouse, utilizing a closed container. The duration of study is two months. Various parameters were recorded. All the data were analyzed and subjected to one-way Anova.
Results: The statistical analysis shows that T3 and T5 exhibited significantly (p<0.05) superior growth rates compared to other treatments. Intriguingly, even when compared against the laboratory control group (T1, T4 and T5), T3 demonstrated markedly better performance in terms of growth rate.
Conclusions: Riccia fluitans can be directly planted into farm environments without acclimatization processes in a laboratory setting
Keywords
Aquatic plants, acclimatization, tissue culture, Riccia fluitans
Status: Abstract Accepted (Oral Presentation)