| The effects of Lactobacillus salivarius and Saccharomyces uvarum on growth performance and immune system against Aeromonas hydrophila Infection in Common carp (Cyprinus carpio) |
| Paper ID : 1022-ICIAQUA |
| Authors |
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Seyedeh Fatemeh Mousavi *1, Delaram Eslimi-Esfahani1, Ehsan Ahmadifar2, Mehdi Ahmadifar1 1Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran 2Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran |
| Abstract |
| Purpose:This study aimed to evaluate the effects of using Saccharomyces uvarum (Su) and Lactobacillus salivarius (LS) in Common carp (Cyprinus carpio) on growth performance and some immunological parameters as well as resistance against Aeromonas hydrophila. Method: A total of 240 fish (~ 5-7g) were randomly distributed in 12 tanks with 20 fish per tank (4 treatments with 3 replications). Four diets were prepared including control diet and three diets supplemented with 107 CFU/g LS, 10 g/kg Su, and LS+Su (107 CFU/g + 10 g/kg). Result: After 45 days, fish fed LS+Su had significantly higher final body weight, weight gain, and specific growth rate compared to control group (P<0.05). The challenge test with A. hydrophila revealed that fish fed LS+Su supplemented diet had highest survival rate. Evaluation of the serum antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD)) activity showed significant (P < 0.05) increase in fish fed both LS and Su as compared to the control. No marked difference was seen in serum total Ig (P > 0.05). Significant increase of IL-1β expression was observed in fish fed LS+Su compared other treatments (P < 0.05). IL-8, TNF-a, lys and TGF-β genes were noticeably up-regulated in the mixture treatments compared to the control group (P < 0.05). No significant difference was observed in IL-10 gene expression between treatments (P > 0.05). Conclusion: This experiment demonstrated the potentially combination effects of LS and Su on growth performance, mucosal immunity, and disease resistance against A. hydrophila infection in Common carp. |
| Keywords |
| Mucosal immunity, Growth performance, Probiotics, disease resistance |
| Status: Abstract Accepted (Oral Presentation) |