| Efficacy of three anesthetics in a cold water fish |
| Paper ID : 1028-ICIAQUA |
| Authors |
|
mohammad farhangi *1, amir hosein zorman2, Hosna Gholipour Kanani3, hosein adineh2 1GONBAD KAVOUS UNIVERSITY 2Gonbad Kavous University 3Gonbad Kavous universtiy |
| Abstract |
| Fish anesthesia is an important step before fish handling in aquaculture and veterinary research fields in order to ease handling and minimize stress and injury of fish. The use of anesthetics to reduce stress and mortality is common in fish farming. To choose an anesthetic, it is necessary to take into account its economic viability, practicality, and effectiveness in use. However, we selected three anesthetics, Nano emulsions of clove oil, nanoemulsions lemonbeebrush-clove oil, and Tricaine Methanesulfonate to test the efficacy in anesthetization of rainbow trout Oncorhynchus mykiss. The experiments were done by 120 fish with average weight 50 ± 12 g. The fish were divided into 3 treatments (each consisting of 10 fish). 3 treatments with 3 replicates were exposed to NBLC and NCL with 10 mg L-1 concentrations and MS with 10 mg L-1 by immersion method. The time of each sedation anesthetics and recovery stages were 24 hours. Our experiment results show that NBL (lemonbeeprush + Clove Nano Essentials Oil) can significantly increase oxygen and reduce ammonia concentration compared to the control (p< 0.05). Some metabolic factors (urea-sodium-potassium) were significantly between different experimental and control, as their highest was obtained in control. The result of this experiment clearly indicated that, NCL had a less time of sedation anesthetics to others, without any mortality.We revealed, a briefly compares of Nano emulsions to MS in this study. |
| Keywords |
| Mortality, Methanesulfonate, Anesthesia, Oncorhynchus mykiss |
| Status: Abstract Accepted (Poster Presentation) |