| Skin mucosal immune parameters changes of Rainbow trout (Oncorhynchus mykiss) Effects of Dietary polyphenol |
| Paper ID : 1058-ICIAQUA |
| Authors |
|
Rudabeh Rufchaie *1, Seyed Hossein Hoseinifar2, shiva Nedaei3 11Iranian Fisheries Science Research
Institute, Inland Waters Aquaculture
Research Center, Agriculture Research
Education and Extension Organization
(AREEO), Bandar Anzali, Iran 2Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran 32Department of Fisheries Science, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran |
| Abstract |
| Purpose: Nowadays, administration of natural immunostimulants, such as medicinal herbs, and functional feed additives are increasing as an alternative practice for antibiotics and other hemotherapeutics. Method :Thus, The present study was conducted to investigate the effects of dietary polyphenol Olive leaf (POL) on skin mucus of Rainbow trout (Oncorhynchus mykiss). During 8 weeks culture period, 144 fish (6.4 ± 0.5 g) were fed by experimental diets containing 0% (control), 0.05%, 0.1% and 0.2 % POL (four treatments in triplicate). In this regard, the mucus sample was employed for evaluation of the mucosal immune parameters. Lysozyme (LYZ) activity was measured by turbid-metric assay described by Ellis, 1990 and The total immunoglobulin (Ig) was determined according to Siwicki 50 μL of the mucus sample was mixed with 50 μL of Gram-positive Micrococcus lysodeikticus, lyophilized cell, (Sigma) suspension and incubated at 30 °C. Equal volume of plasma and polyethylene glycol solution (50 μL) were mixed together, shake and then centrifuged to precipitate Ig. Results: At the end of the feeding trial, the fish fed with the highest POL concentration in their diets (0.2% POL) exhibited significantly higher skin mucus LYZ and Ig values compared to the control (p < 0.05). that skin mucus parameters could be widely influenced by environmental and nutrition modification. the present study demonstrated that the polyphenol Olive leaf at optimum inclusion levels could significantly improve skin mucus innate immunity through modulation of, Ig, Lysozyme . Conclusions: The improvement of mucosal LYZ activity in the present study might be due to the immunomodulatory effects of water hyacinth to stimulate skin-associated lymphoid tissues, which in turn could boost immune capability against possible stressors. the present study shows that dietary administration of POL positively affects mucosal immunity of Rainbow trout . |
| Keywords |
| polyphenol , Rainbow trout, Skin mucosal, immune parameters |
| Status: Abstract Accepted (Poster Presentation) |