A new synbiotic consisting Lactobacillus casei and banana peel powder as supplementation in zebrafish (Danio rerio) diet can improve growth and innate immune responses
Paper ID : 1059-ICIAQUA
Authors
Hamideh Zakariaee *1, Mohammad Sudagar1, Zohreh Khorshidi1, GHASEM ASHOURI2
1Department of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2Department of Sciences and Technologies, University of Sannio, Via De Sanctis, snc, 82100 Benevento, Italy
Abstract
Purpose: An In vivo study was conducted to improve growth performance and some immunity parameters in zebrafish (Danio rerio) larvae.
Method: To promote the immunity factors and growth indices, zebrafish larvae fed with supplementary diets with a new synbiotic consisting Lactobacillus casei as probiotic and banana peel as prebiotic sources. The larvae (60 ± 1.0 mg) were fed for 8-week period on different supplementation diets as follows: Control (basal diet), 1% banana peel powder (prebiotic diet), 107 CFU g-1 L. casei (probiotic diet), 107 CFU g-1 L. casei + 1% banana peel powder (synbiotic diet). At the end of the trial, growth performance, survival and innate immune responses were evaluated by completely random sampling.
Results: Growth performance was significantly improved in fish fed with supplemented diets (P < 0.05). No significant differences of survival were observed between the different treatments as compared to control (P > 0.05). Furthermore, innate immune parameters were enhanced in fish fed diet supplemented synbiotic in comparison with control group (P < 0.05).
Conclusions: Altogether, these results indicated that L. casei combined with banana peel powder as an efficient symbiotic can improve the growth and innate immune parameters in zebrafish (Danio rerio).
Keywords
Zebrafish, Banana, Lactobacillus casei, growth, immunity.
Status: Abstract Accepted (Oral Presentation)