| Effect of earthworm Eisenia foetida extract on Hematology analysis of Caspian roach Rutilus caspicus |
| Paper ID : 1063-ICIAQUA |
| Authors |
|
Rudabeh Rufchaie * 1Iranian Fisheries Science Research
Institute, Inland Waters Aquaculture
Research Center, Agriculture Research
Education and Extension Organization
(AREEO), Bandar Anzali, Iran |
| Abstract |
| Purpose: The earthworm species Eisenia foetida is recognized for its high content of amino acids, unsaturated fats, and omega-3 fatty acids. In light of this, our study aimed to assess the impact of earthworm extract (EE) supplementation on various parameters including feed consumption, growth metrics, immune responses, and stress resilience in Caspian roach fingerlings. Method :We designed three experimental groups with triplicate tanks: two with different concentrations of EE mixed in distilled water at ratios of 1:25 (T1) and 1:50 (T2), and a third group receiving a control diet consisting of distilled water without EE. The fish (average weight 4.30 ± 0.10 g) were housed in nine 100-liter tanks. At the end of the rearing, the blood was drawn from the caudal stem, then poured into vials containing heparin and tested by standard laboratory methods. Data were compared using one-way ANOVA and Duncan's test. Results: The results showed that the 1:50 treatment had the highest mean red blood cell (1343333/3±40414/5 n/ml) And white blood cells (59000 ± 2000 n/ml) was reported) p < 0.05) . Hematocrit percentage and hemoglobin concentration between 1: 50 treatments( 67.3 ± 0.5 % and 8.1±0.1g/dl) And treatment 1:25 ( 56.6 ± 0.5% and 8.2± 0.1 g/dl) There was no significant difference between the two treatments but the difference between the treatments supplemented with the control treatment was significant) p < 0.05). Estimated percentages of lymphocytes, monocytes and neutrophils showed no significant differences between treatments (p> 0.05). Conclusions: Overall, the data suggest that inclusion of EE in the diets of Caspian roach fingerlings has beneficial effects and could be considered for use in aquaculture practices for this species. Moreover, the group receiving the earthworm extract at a dilution of 1:25 V/V demonstrated superior outcomes |
| Keywords |
| Eisenia foetida, Hematology analysis, Rutilus caspicus |
| Status: Abstract Accepted (Poster Presentation) |