| Effect of water hyacinth leaf on antioxidant activity common carp |
| Paper ID : 1066-ICIAQUA |
| Authors |
|
Rudabeh Rufchaie *1, Mohadeseh Ahmadnezhad2 11Iranian Fisheries Science Research
Institute, Inland Waters Aquaculture
Research Center, Agriculture Research
Education and Extension Organization
(AREEO), Bandar Anzali, Iran 21Iranian Fisheries Science Research Institute, Inland Waters Aquaculture Research Center, Agriculture Research Education and Extension Organization (AREEO), Bandar Anzali |
| Abstract |
| Purpose: Water hyacinth is rich in bioactive compounds like phenolic compounds, flavonoids, alkaloids, and terpenoids, which have antimicrobial and antioxidative properties. Research has focused on using water hyacinth leaf powder (WLP) as a feed additive for common carp, a fast-growing freshwater fish with high flesh quality and economic value, to improve growth, immune response, and antioxidant activity. Method :During a seven-week investigation, scientists evaluated the effects of different levels (0, 0.5, 1, and 1.5%) of (WLP) incorporated into the diet of common carp.One hundred and twenty fish, each averaging a weight of 50.07 ± 0.79 grams, were allocated randomly into four separate groups. At the conclusion of the trial, fish from each group were selected randomly post a 24-hour fasting period. Their blood was then collected, pooled, and placed immediately into tubes without anticoagulant agents to allow clotting at room temperature. The resulting serum was preserved at −20°C for subsequent analysis. The SOD activity was quantified using the method described by Marklund and Marklund, which involves determining the quantity of enzyme necessary to inhibit the auto-oxidation of pyrogallol. GPX activity was determined following Günzler's protocol, which measures NADPH oxidation via glutathione reductase in a coupled reaction. CAT activity was gauged based on the rate of hydrogen peroxide reduction as outlined by Goth. Method :At the end of the feeding trial, results revealed a significant increase in catalase (CAT) activity was detected in WLP supplemented groups, while higher superoxide dismutase (SOD) activity was detected in 0.5 and 1.5% WLP groups (P<0.05). However, glutathione peroxidase (GPx) activity was higher only in 0.5% WLP group (P<0.05). Conclusions:This study conclusively demonstrates that the oral intake of water hyacinth enhances the antioxidant capabilities in C. carpio, suggesting that its use as a phytomedicine in aquaculture could potentially mitigate its negative economic and ecological effects. |
| Keywords |
| common carp, water hyacinth, antioxidant activity |
| Status: Abstract Accepted (Poster Presentation) |