| Synergistic Effects of Dietary Selenium and Pediococcus acidilactici on non-Specific Immune Response in Rainbow Trout (Oncorhynchus mykiss) |
| Paper ID : 1082-ICIAQUA |
| Authors |
|
Masoud Kousha *1, mehdi mohammadalikhani2, Sakineh Yeganeh3, Abdolsamad Keramat3 1Coldwater Fisheries Research Center (CFRC), Iranian Fisheries Sciences Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), 46733-11802 Tonekabon, Iran 2Coldwater Fisheries Research Center (CFRC), Iranian Fisheries Sciences Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), 46773-11802 Tonekabon, Iran 3Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Km 9 Darya Boulevard, P.O. Box: 578, Sari, Iran |
| Abstract |
| Purpose: Role of selenium in the enhancement of fish growth and health is not only relying on its amount, but also on the transformation of digested selenium to metabolically more active forms. Lactic acid bacteria (LAB) can transform inorganic selenium into organic selenium species. The benefits of LAB can also be improved in the presence of inorganic selenium. Therefore, the aim of this study was to investigate the synergistic effects of dietary sodium selenite and P. acidilactici on non-specific immune response in rainbow trout (Oncorhynchus mykiss). Method: A 3 × 3 factorial design experiment was conducted with nine purified diets including three levels of sodium selenite (0, 1 and 2 mg/kg) and three levels of P. acidilactici (0, 7 and 9 log CFU/g) for 8 weeks. Five hundred and forty rainbow trout with an average initial body weight of 30 ± 2 g were randomly distributed into 27 plastic tanks of 140 L. Blood samples were taken from three fish per tank. For the lysozyme assay, obtained fish serum was added to phosphate‐buffered saline containing Micrococcus lysodeikticus. Absorbance at 530 nm was measured for 4 min. One unit of lysozyme activity was determined as a reduction in absorbance of 0.001 per min. Serum complements C3 and C4 activity was also determined by using specific assay kits according to manufacturer's instructions obtained from Nanjing, China. Results: Lysozyme activity was raised by increasing dietary P. acidilactici levels in all dietary selenite levels, whereas lysozyme activity was increased by increasing selenite levels in the presence of dietary P. acidilactici (p < .05). Serum C3 and C4 activity were also increased by increasing dietary P. acidilactici levels in all dietary selenite levels (p < .05). However, C3 activity was increased by increasing dietary selenite levels in the absence of dietary P. acidilactici, and C4 activity was increased by increasing dietary selenite levels in the 0 and 7 log CFU/g P. acidilactici (p < .05). Conclusions: The most synergistic effects of dietary supplements on non-specific immune response of rainbow trout were obtained at 2 mg/kg sodium selenite and 7 log CFU/g P. acidilactici. |
| Keywords |
| Sodium selenite (Na2SeO3); Lactic acid bacteria (LAB); Pediococcus acidilactici; Rainbow trout; Oncorhynchus mykiss. |
| Status: Abstract Accepted (Poster Presentation) |