| The effect of chilled storage conditions on sperm quality of the Anzali lagoon migratory population of the Caspian roach (Rutilus caspicus) |
| Paper ID : 1088-ICIAQUA |
| Authors |
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Erfan Akbari Nargesi * Inland Water Aquaculture Research Centre, Iranian Fisheries Science Research Institute, Agriculture Research Education and Extension Organization, Bandar-e Anzali, Iran |
| Abstract |
| Chilled storage of fish sperm is a practical strategy that can efficiently facilitate fertilization procedures in hatchery conditions. In this experiment, the percentage of motile sperm and motility time of the Caspian roach (Rutilus caspicus) were investigated at 0, 24, 48, 72, 96, 120, 144, and 168 hours after chilled storage at 4 °C. For this purpose, sperm of eight mature males with a mean weight of 54.3 ± 1.9 g was used. The fish were anesthetized with clove powder extract, sperm was then collected by gentle pressure to the abdominal region and stored in plastic tubes under chilled conditions. After 48 hours of chilling storage, there was a significant decrease in the percentage of motile sperm and motility time compared to the 0 time (P < 0.05). All sperm samples were active up to 72 hours after chilling storage with the mean activity and motility time of 46.2 ± 11.9% and 40.1 ± 4.5s, respectively. Following 144 hours of storage, the mean activity in the 3 remaining samples was 7.5 ± 11.6%. After 168 hours, no activity was observed in the remaining samples. Based on the results, samples that are stored under chilled conditions for up to 48 hours could be suitable for the fertilization process. Consequently, chilled storage of the Caspian roach sperm for up to 48 hours could facilitate the artificial reproduction process in the hatchery centers. |
| Keywords |
| Artificial reproduction, broodstock management, Cyprinidae, Motility, Semen |
| Status: Abstract Accepted (Poster Presentation) |