| Sperm quality of wild common carp (Cyprinus carpio) under chilled storage conditions |
| Paper ID : 1089-ICIAQUA |
| Authors |
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Erfan Akbari Nargesi *1, Danial Gorouhi2, Alireza Kashani Sabet2 1Inland Water Aquaculture Research Centre, Iranian Fisheries Science Research Institute, Agriculture Research Education and Extension Organization, Bandar-e Anzali, Iran 2Shahid Ansari Teleost Fish Restocking and Genetic Conservation Center, Rasht, Guilan, Iran |
| Abstract |
| Sperm can be stored under chilled conditions for a few hours to several months. Storing sperm can eliminate the need for keeping males and facilitate easier sperm handling when eggs are available. In the present study, motility time and percentage of motile sperm of wild common carp (Cyprinus carpio) at 0, 24, 48, 96, 120, and 144 hours after chilled storage (+4 °C) were investigated. For this purpose, the semen of 6 mature males with a mean weight of 3379.2 ± 1301.9 g was used. First, the fish were anesthetized with clove powder extract, and then sperm were collected by gentle pressure to the abdominal region. One mL of each semen sample was kept in plastic tubes and stored under chilled conditions. Based on the results, significant differences in motility time and percentage of motile sperm were observed at all times after 24 hours of chilled storage compared to the 0 time (P < 0.05). All sperm samples were active for up to 48 hours after chilled storage. At this time, the mean activity and motility time of sperm samples were 36.7 ± 17.5 % and 39.0 ± 5.3s, respectively. According to the results, after 24 hours of chilled storage, the samples were still at the appropriate level of the percentage of motile sperm (68.3 ± 24.8%) and motility time (48.2 ± 7.3s) for artificial fertilization. Consequently, up to 24 hours of chilled storage of wild common carp sperm can facilitate the fertilization process in hatchery centers. |
| Keywords |
| Controlled reproduction, Cyprinidae, Fertilization, Motility, Semen |
| Status: Abstract Accepted (Poster Presentation) |