| Efficacy of injectable killed polyvalent vaccines against vibriosis and streptococcosis infections in Asian seabass (Lates calcarifer) |
| Paper ID : 1099-ICIAQUA (R1) |
| Authors |
|
mina ahangarzadeh *1, Hossein Houshmand2, Mansour Torfi Mozanzadeh3, Samira Nazemroaya4, Ashkan Ajdari5, Lefteh Mohseninejad2 1South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute,
Agricultural Research Education and Extension Organization, Ahvaz, Iran. 2South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Ahvaz, Iran. 3South Iran Aquaculture Research Centre, Iranian Fisheries Science Institute (IFSRI), Agricultural Research Education and Extension organization (AREEO), Ahwaz, Iran 4South Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran 5Offshore Fisheries Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Chabahar, Iran. |
| Abstract |
| This experimental study investigated the efficacy of polyvalent autogenous formalin-inactivated vaccines containing Vibrio harveyi, V. alginolyticus and Streptococcus iniae in Asian seabass. for this purpose, 180 fish were divided into three groups with 60 fish in triplicate: I) a control group injected with phosphate-buffered saline (PBS), II) a group vaccinated by polyvalent vaccine (V. alginolyticus + V. harveyi + S. iniae) and III) a group vaccinated with the same polyvalent vaccine plus an oral booster. Immunological parameters and antibody titer were measured before and at three, five-, and eight-weeks post-vaccination. The challenge test with each isolate was performed five weeks post-vaccination. Use of this polyvalent vaccine by injection and oral booster resulted in an improved specific and non specific immunity in Asian seabass. The highest relative percentage survival rate, 85.71 ± 3.57% was observed in vaccinated group with polyvalent vaccines + oral booster when challenged with V. harveyi. Also, the vaccinated fish produced significantly higher antibody titers against V. alginolyticus, V. harveyi and S. iniae than the control group (P < 0.05). Non-specific immune parameters were significantly enhanced in the vaccinated groups, especially group III, compared to the control. The results demonstrated that the administration of a killed polyvalent vaccine can effectively protect Asian seabass against V. alginolyticus, V. harveyi and S. iniae. |
| Keywords |
| killed polyvalent vaccine, specific immunity, non specific immunity, V. alginolyticus, V. harveyi , S. iniae, Asian seabass |
| Status: Abstract Accepted (Poster Presentation) |