| The effect of Lactobacillus plantarum and Bacillus subtilis probiotics on Persian Gulf Sea Bream (Acanthopagrus latus) Immunity |
| Paper ID : 1100-ICIAQUA (R1) |
| Authors |
|
Saman Ahani *1, Ali Taheri Mirghaed2, Sara Ahani1 1Department of Veterinary Science, Islamic Azad University, Karaj Branch, Karaj, Iran 2Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran |
| Abstract |
| Purpose: The present study investigated the immunostimulatory effects of two probiotics in 20 gram Persian Gulf Sea bream (Acanthopagrus latus). Method: Experimental treatments included Bacillus subtilis, Lactobacillus plantarum, a combination of these two probiotics and a control treatment (without probiotics), which were added as a spray to the basal diet (marine fish feed provided by Beyza21® Company). After the adaptation period, the fish were fed with the experimental treatments four times a day to satiation for 90 days. Results: The results demonstrated that by adding probiotics, both serum lysozyme activity (600 unit/ml) and mucosal lysozyme activity (575 unit/ml) significantly(P<0.05) improved in the group treated with Bacillus subtilis compared to the control group (420 and 310 unit/ml, respectively, in serum and mucosa). It indicates the enhancement of the immune response to bacterial infections. Catalase activity also decreased from 31 U/mg in the control group to 16 U/mg of protein in the third treatment containing a mixture of two probiotics. This significant decrease (P<0.05) indicates reduced oxidative stress and improved liver health. Additionally, the survival rate in fish fed with the third treatment containing a mixture of the two probiotics significantly increased(P<0.05) compared to the control group and was equal to 93%. The survival rates in other experimental groups also showed a slight increase (P>0.05) compared to the control group. Conclusions: The results of the present study indicate that adding Bacillus subtilis and Lactobacillus plantarum probiotics can enhance immunity in Sea bream and significantly increase the survival rate in the industry of cage culture. |
| Keywords |
| Sea bream, Probiotics, Marin fish, Lactobacillus plantarum, Bacillus subtilis |
| Status: Abstract Accepted (Poster Presentation) |