| Comparison of various physical methods (freezing and thawing, homogenization and ultrasound) in extracting phycocyanin pigment from Spirulina platensis |
| Paper ID : 1108-ICIAQUA |
| Authors |
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Nasrollah Ahmadifard *1, Arezoo Naemi2, Saeideh Mahasen kahag2 111th km of SERO Blvd. Urmia, West Azarbayjan, Iran. Postal code: 5756151818 2Department of Fisheries, Faculty of Natural Resources, Urmia University, P.O. Box: 57153-165, Urmia, Iran. |
| Abstract |
| Spirulina platensis is one of the photoautotrophic and blue-green single-celled microalgae, whose cultivation has been developing in recent years. This algae with high digestibility has various amounts of bioactive compounds such as pigments, proteins, unsaturated fatty acids, antioxidants and vitamins with wide applications in feed as well as food supplements, industries, cosmetics, sewage treatment, etc. Phycocyanin is a non-toxic and water-soluble protein pigment from microalgae that has antioxidant, anti-inflammatory, hepatoprotective and fluorescent properties. Extracting pigments from cyanobacteria is not easy due to the specificity of their multi-layered cell walls. For this purpose, the cell wall of the algae must be broken first. There are various methods to break the cell wall and release phycocyanin from the cell. The purpose of this study was to investigate the amount of extraction of phycocyanin protein pigment by various physical methods (freezing and thawing cycles, homogenization and ultrasound). First, Spirulina were cultured in Zarouk culture medium, and the number of trichomes increased from 14 ± 0.8 to 103 ± 0.8 per ml in 22 days. This number of algae showed a growth rate equal to 0.09 per day. Based on the results, the highest amount of phycocyanin pigment extraction was related to the freezing and thawing method. In the freezing and thawing method, the values of 0.76, 0.4 and 0.04 mg/ml of phycocyanin were obtained in the first to third stages, respectively. In the homogenization and ultrasound methods, the values of 0.24 and 0.37 mg/ml of phycocyanin were obtained, which were lower than the freezing and thawing methods. Based on the results, it was found that the use of freezing and thawing cycles is more effective in extracting phycocyanin pigment despite the long process. |
| Keywords |
| Spirulina, algae, phycocyanin pigment, freezing and thawing, homogenization and ultrasound |
| Status: Abstract Accepted (Poster Presentation) |