| Optimum enrichment level of adult Artemia franciscana with β-carotene extracted from algae Dunaliella salina |
| Paper ID : 1112-ICIAQUA |
| Authors |
|
Nasrollah Ahmadifard *1, Yaser abdollahi2 111th km of SERO Blvd. Urmia, West Azarbayjan, Iran.
Postal code: 5756151818 2Department of Fisheries, Faculty of Natural Resources, Urmia University, P.O. Box: 46414-356, Urmia, Iran |
| Abstract |
| This study was conducted to determine the optimum time and concentration of β-carotene extracted from algae Dunaliella salina for the enrichment of adult Artemia franciscana based on the intestinal filling, total carotenoid, and mortality rate. The β-carotene was used as a solution in castor oil at a concentration of 4%. The enrichment emulsion was prepared using one gram of oil containing β-carotene and 0.2 grams of soybean lecithin at a ratio of 5 to 1 in 10 ml of water at 40 °C. To do this, 36,000 adults A. franciscana were transferred to 12 conical glass container (3,000 per each one). The emulsion was added at 2, 4, 6 and 8 ml L-1 to the enrichment vessel and maintained in 2, 4, and 6 hours. After the end of enrichment period, the number of dead Artemia was counted and, the mortality percentage was calculated. Also, 20 individual of enriched Artemia were collected from each container and, using a microscope their intestinal filling was measured. Finally, the amount of total carotenoids in enriched Artemia was determined. Based on the results of intestinal filling, the maximum amount of Artemia enrichment was significantly obtained using 8 mg L-1 of the emulsion at 2 and 4 hours (p <0.05). The highest total carotenoid of the enriched Artemia was obtained at 6 mg L-1 of emulsion and 4 hours without any significant differences with higher values and longer duration. In addition, lower Artemia mortality rate was significantly observed in the duration of 4 hours and the amount of 6 mg L-1 of emulsion compared to the longer duration and the higher amount of emulsion (p <0.05). In conclusion, the duration time and the amount of enrichment emulsion depend on the nature of the substance to form liposomes. Therefore, for studies on Artemia enrichment, it is essential to determine the optimal level of different materials. |
| Keywords |
| Enrichment, Artemia franciscana, β-carotene, Algae Dunaliella salina |
| Status: Abstract Accepted (Poster Presentation) |